Sunday, 20/08/2017

PROTRANS HLA SBT

Sequencing



PROTRANS S4

Sequencing Strategy

  • Allele - and Allele-Group specific Amplification Primer dried in PCR Strips
  • Splitting the DNA in two Haplotypes and Sequencing each Haplotype separately
  • No Ambiguities, because the Haplotypes are amplified and sequenced separately
  • Only one Mastermix per DNA
  • Identical program for Amplification and Sequencing for:

    HLA-Class I:     HLA-A, HLA-B, HLA-C
    HLA-Class II:    HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,
                                HLA-DQB1, HLA-DQA1, HLA-DPB1

  • Software generates the Plate Record automatically
  • Low volume or high throughput - manually or full automated
  • Safety through automation
  • Detection and definition of all new HLA-Alleles
  • Results in only one Day
  • Complete Exons and easy automated analysis of Sequencing files

 

Haplotype Specific Sequencing

Publications concerning SBT

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