Sunday, 20/08/2017

PROTRANS HPA SSP

Human Platelet Alloantigens (HPA) can induce the formation of alloantibodies in individuals
lacking the corresponding antigens. These antibodies have been demonstrated to play an
important role in clinical conditions like neonatal alloimmune thrombocytopenia,
posttransfusion purpura and platelet transfusion refractoriness indicating the necessity
of a well match between parents and children or donor and recipient.

As HPA Phenotyping is often limited to the rare availability of reliable HPA antisera,
insufficient amount of platelets or disturbing HLA class I antibodies,
HPA SSP Genotyping should be the method of choice.

Using the Protrans HPA SSP Domino System HPA genotyping can be performed
with genomic DNA extracted from small amount of cellular material to
identify the present HPA antigens.


Table1: HPA antigens and frequencies in caucasians

System

Antigen

Phenotype Frequency

Glycoprotein

HPA-1

HPA-1a

97.9%

GPIIIa

HPA-1b

28.8%

HPA-2

HPA-2a

>99.9%

GPIb

HPA-2b

13.2%

HPA-3

HPA-3a

80.95%

GPIIb

HPA-3b

69.8%

HPA-4

HPA-4a

>99.9%

GPIIIa

HPA-4b

<0.1%

HPA-5

HPA-5a

99.0%

GPIa

HPA-5b

19.7%

HPA-6

HPA-6a

99,5%

GPIIIa

HPA-6b

0.7%

HPA-15

HPA-15b

60.2%

CD109

HPA-15a

80.5%




Advantages
  • Only one pipetting step to define the HPA Antigens: HPA1a/1b, HPA2a/2b,
    HPA3a/3b, HPA4a/4b, HPA5a/5b, HPA6a/6b and HPA15a/15b
  • Report of HPA Antigens in less than 2 hour using the
    Protrans electrophoresis equipment

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